Daniel J. Guillaume, MD, MS

Associate Professor and Director of Quality and Safety, Department of Neurosurgery

Daniel J. Guillaume

Contact Info


Mailing Address:
MMC 96
420 Delaware St. SE
Minneapolis, MN 55455

Administrative Assistant Name
Deborah Fellows

Administrative Phone

Administrative Email

Associate Professor and Director of Quality and Safety, Department of Neurosurgery

Associate Professor, Department of Pediatrics


University of Iowa Carver College of Medicine

University of Iowa Hospitals and Clinics (Neurosurgery)

Prince of Wales Hospital and Sydney Children's Hospital, (Pediatric and Minimally Invasive Neurosurgery)

University of Iowa (Neurosurgery)

University of Wisconsin (Stem Cell Research)


After completing his neurosurgery residency at the University of Iowa Hospital and Clinics, Dr. Guillaume completed two clinical fellowships. The first was at the University of Iowa where he worked with Dr. Arnold Menezes, a pediatric neurosurgeon and world expert in development and pathologies of the cranial base and craniovertebral junction. He then completed a fellowship at the Centre for Minimally Invasive Neurosurgery, Prince of Wales Hospital and Sydney Children's Hospital in Sydney, Australia with Dr. Charles Teo, a pediatric neurosurgeon and pioneer in minimally invasive approaches to the brain. These approaches allow treatment of problems, including hydrocephalus and brain tumors, through a tiny incision, often leading to less pain and a shorter hospital stay after surgery. In many cases, these approaches can successfully treat hydrocephalus with avoidance or removal of shunt catheters, and some brain tumors can be removed through the nose or eyebrow avoiding the need for large scalp incisions.

Through his unique training, Dr. Guillaume has developed expertise for managing pediatric brain tumors and hydrocephalus, Chiari malformation, and pediatric skull base surgery to name a few. He joined the University of Minnesota in 2012 after practicing pediatric neurosurgery for almost six years as a faculty member at Oregon Health & Science University in Portland. He has developed experience in caring for children with all types of congenital and acquired neurosurgical problems.

Dr. Guillaume is currently Chief of Pediatric Neurosurgery at the University of Minnesota Masonic Children's Hospital and Director of the Division of Pediatric Neurosurgery for the Department of Neurosurgery. His interests include advanced treatments of pediatric brain and spinal tumors, minimally invasive surgical approaches, complex hydrocephalus, and Chiari malformation. He has published in numerous journals and books, directed courses on minimally invasive and endoscopic surgical approaches, served as reviewer and co-editor for major neurosurgery and stem cell journals and served as a moderator and scientific program committee member for national pediatric neurosurgical meetings.

Dr. Guillaume was driven toward pediatric neurosurgery because he enjoys caring for children and their families, and is committed to improving the lives of children suffering from neurological problems.

Awards & Recognition

  • MplsStPaul Magazine’s Top Doctors, 2013, 2014, 2015, 2016, 2017
  • Best Doctors in America, 2011- 2012; 2013, 2014, 2015, 2016, 2017
  • Best Oregon Doctors, Portland Monthly Magazine, 2006, 2007, 2008
  • SuperDocs, 2013, 2014, 2015, 2016
  • Top Neurosurgeon in Minneapolis, MN, International Association of Healthcare Professionals 
  • The Leading Physicians of the World, 2014, 2015, 2016

Professional Associations

  • Pituitary Society, 2016 to present
  • American Society of Pediatric Neurosurgeons, 2013 to present
  • Minnesota Neurosurgical Society, 2012 to present
  • American Academy of Pediatrics, 2012 to present
  • International Society for Pediatric Neurosurgery, 2011 to present
  • International Brain Barriers Society, 2008 to present
  • Society for Neuro-oncology, 2008 to present
  • Hydrocephalus Association, 2008 to present
  • AANS/CNS Section of Pediatric Neurosurgery, 2007 to present
  • Children’s Oncology Group, 2007 to present
  • American Association of Neurological Surgeons, 1999 to present
  • Congress of Neurological Surgeons, 1999 to present


Research Summary/Interests

Research Interests

Dr. Guillaumes’s research is focused on improving diagnosis and therapy for pediatric brain tumors by use of advanced imaging, and efforts to improve delivery of anti-tumor agents by manipulation of the blood-brain barrier. His other ongoing research studies evaluate methods to improve treatment of hydrocephalus using image guidance, and investigate the association of hearing loss in treatments for hydrocephalus.

Research Projects

  • Evaluation of hearing loss in children after treatment for hydrocephalus, Funded by the Capita foundation
  • Study of treatments for Chiari I malformation with syringomyelia (member of the Park-Reeves Syringomyelia Research Consortium), funded by the Park-Reeves Foundation
  • Imaging vascular properties of pediatric brain tumors using DSC-MRI with ferumoxytol (Code 7228) and DCE MRI with gadolinium in a single imaging session: An NCI sponsored exploratory trial, Funded by NIH (OHSU, Portland, OR).
  • A Randomized, Controlled, Multicentre, Phase IIb Safety and Efficacy Study of HGT-1410 (Recombinant Human Heparan N Sulfatase) Administration via an Intrathecal Drug Delivery Device in Pediatric Patients with Early Stage Mucopolysaccharidosis Type III A Disease, Jan 2015 to present, Role:Co- investigator (PI- C Whitley), agency: Shire
  • AAVrh.10-hMPS3A, an adeno-associated viral (AAV) vector encoding the human N-sulfoglycosamine sulphohydrolase (SGSH) gene, Jan 2015 to present, Role:Co- investigator (PI- C Whitley), agency: Lysogene



Impact Analytics

Total Publications
First/Last Author Publications
Total Citations

Peer-Reviewed Publications

  1. Hoshide R, Melzer H, Dalle-Ore D, Gonda D, Guillaume D and Chen CC. Impact of ventricular-peritoneal shunt valve design on clinical outcome of pediatric patients with hydrocephalus: Lessons from randomized controlled trials. Surg Neurol Int. 2017 Apr 5; 8:49. PMID: 28480111
  2. Miller C, Guillaume D, Dusenberry K, Clark B, Moertel C. Report of effective trametinib therapy in two children with progressive hypothalamic optic pathway pilocytic astrocytoma: documentation of volumetric response. J Neurosurg Peds, 2017 Mar; 19(3):318-324 Epub 2016 Dec 23. PMID 28009226. Impact Factor: 1.70
  3. George CL, Harper NS, Guillaume D, Cayci Z and Nascene D. Vascular Channel Mimicking a Skull Fracture. J Pediatr, 2017 (181)325-326.e1. PMID 27856000. Impact Factor: 1.49
  4. Svalina MN, Kikuchi K, Settelmeyer TP, Abraham J, Lal S, Davare MA, Young MC, Peckham JL, Cho Y, Michaelek JE, Hernandez BS, Berlow NE, Jackson M, Guillaume DJ, Selden NR, Bigner DD, Nazemi KJ, Green SC, Corless CL, Gultekin S, Mansoor A, Rubin BP, Woldjer R, Keller. IGF-1R as a key target in high risk, metastatic medulloblastoma. Scientific Reports. 2016 Jun 3;6:27012. PMID: 27255663. Impact Factor: 5.47. Cited: 1
  5. Miller C, Guillaume D. Management of midline dural sinus malformations and review of the literature. Child’s Nervous System, 2016, 32(8):1449-1461. PMID: 27207610Impact Factor: 0.62
  6. Miller C, Guillaume D. Incidence of hemorrhage in the pediatric population with placement and removal of external ventricular drains.J Neurosurg Pediatr. 2015 Dec; 16(6):662-7. Epub 2015 Sep 18. PMID: 26382184. Impact Factor: 1.75
  7. Satzer D, Guillaume DJ. Hearing loss in hydrocephalus: a review, with focus on mechanisms. Neurosurg Rev. 2016 Jan,39(1):13-24. Epub 2015 Aug 18. PMID: 26280639Impact Factor: 2.16
  8. Jagadeesan BD, Grande AW, Guillaume DJ, Nascene DR, Tummala RP. The role of percutaneous embolization techniques in the management of dural sinus malformations with atypical angio-architecture in neonates: report of 2 cases. J Neurosurg Pediatr. 2015 Jul;16(1):74-9. Epub 2015 April 24. PMID: 25910034. Impact Factor: 1.757
  9. Das P, Guillaume DJ. Targeting the RAS-ERK signaling pathway as therapy for pediatric brain tumors: A review. Journal of Pediatric Oncology, 2015, 3:1-7. Impact Factor: 1.146
  10. Satzer D, Guillaume DJ. Hearing loss in hydrocephalus: a review, with focus on mechanisms. Neurosurgical Review, 2015 Jan:39(1):13-24. Impact Factor: 2.166. Dr. Guillaume mentored the first author of the paper (a medical student) during the entire project from writing the IRB to paper submission and played a key role in manuscript preparation, editing, review and submission.

Book Chapters

  • Das P and Guillaume D. Pediatric Brain Tumors: Other, in: Neurosurgical Knowledge Update, editors: Mark Krieger and Gerry Grant, Thieme, In press
  • Guillaume DJ. Congenital Defects of the Scalp and Skull, in: Pediatric Neurosurgery Tricks of the Trade (Ed: Alan Cohen). Thieme, in press.
  • Guillaume DJ, Doolittle ND, Neuwelt EA. Oligodendroglial Tumors: Intra-arterial chemotherapy, in “Tumors of the CNS”, E. Hayat (ed), In Press, 2011.
  • Guillaume DJ. Minimally invasive neurosurgery for CSF Disorders, in Neurosurgery Clinics of North America, 21(4)653-672, 2010, PMID: 20947034.
  • Guillaume DJ and Menezes AH. Hydrocephalus in Chiari malformation and other craniovertebral junction abnormalities, in Mallucci C and Sgouros S (editors) “Cerebrospinal Fluid Disorders” Informa Healthcare, NY; pp 300 – 317, 2009.
  • Guillaume DJ and Teo C. Endoscopic management of specific disease entities, in Mallucci C and Sgouros S (editors) “Cerebrospinal Fluid Disorders” Informa Healthcare, NY; pp 508 – 527, 2009.
  • Guillaume DJ. Chiari Malformation and Syringomyelia, Pediatric Neurosurgery, Iskandar B (ed) (educational series of the an Arab Journal of Neurosurgery), MSD Printing Press, pp 238 – 258, 2008.
  • Guillaume D, Haddad F, Haddad G and Hitchon P. Diagnosis and management of traumatic intracranial hemorrhage, in Schmidek H and Roberts D (editors) “Operative Neurosurgical Techniques” 5th ed, pp 1224 – 1232, 2005.
  • Guillaume D and Hitchon PW. Medical and surgical management of intracerebral hemorrhage, in Adams HP (editor) “Handbook of cerebrovascular disease,” 2nd edition pp 477-5000 (chap 21), 2004.
  • Guillaume D, and Zhang SC. Neuronal replacement by transplantation, in Bottenstein JE (editor) “Neural Stem Cells: Development and Transplantation,” page 307-338, 2003.
  • Guillaume D, Karanjia PN, and Hitchon PW. Traumatic intracranial aneurysms. Medlink Neurology (online information resource for clinical neurology), www.medlink.com, 2003.
  • Guillaume DJ and Hitchon P. Spontaneous intracerebral hemorrhage, in Sinha KK (editor), “Advances in Clinical Neurosciences”, 13th ed, p 501-516, 2003.
  • Guillaume DJ, Haddad GF and Hitchon P. Post-traumatic intracranial aneurysms, in Sinha KK (editor), “Advances in Clinical Neurosciences”, 13th ed, p 517-526, 2003.

Other Published Articles


Presentations, Posters, and Exhibits

  • Mustonen E, Segura B, Guillaume D, Murati M, Wagner S, Nerheim D, Pothoff S, Lawton D, Louie J. Can Pediatric Specialists Change Culture at a General Emergency Department? Reducing Cervical Spine CT Use for Neck Trauma – A Preliminary Report. Minnesota Medical Association, Rochester, MN, September 2017.

Invited Oral Presentations at National and International Professional Meetings

  1. Management of traumatic brain injury in adults and children. Trauma Grand Rounds, Hibbing, MN, March 28, 2017.
  2. Clinical aspects of the blood-CSF barrier. Blood-brain barrier annual meeting, Stevenson, WA, March 2, 2017.
  3. Management of optic pathway/hypothalamic astrocytomas. Children’s Oncology Group Annual Meeting, Atlanta, GA, Oct 15, 2016.
  4. Aspiring to be the best: How to hit a moving target. Neurosafe2016, Chaska, MN, July 16, 2016
  5. Adults with Hydrocephalus diagnosed in young and middle age. Hydrocephalus Association Annual Meeting, Minneapolis, MN, June 20, 2016.
  6. Endoscopic management of complex ventricular tumors. Children’s Oncology Group Annual Meeting, Dallas, TX, Oct 8, 2015
  7. Management of Pineal Region Tumors. Kalinga Hospital, Bhubaneswar, India, June 16, 2015
  8. Endoscopic management of Ventricular Tumors. Kalinga Hospital, Bhubaneswar, India, June 16, 2015
  9. Neurosurgical simulation at the University of Minnesota. NeuroTouch International Consortium Meeting. Montreal, QC, Canada. February 20, 2015.
  10. Neurosurgery in India, University of Minnesota Department of Anesthesia Grand Rounds, December 9, 2014.

Contributed Papers Presented at Professional Meetings, Conferences, etc.

  1. Guillaume DJ, Anderson J, Pollock J and Bardo D. Detection of cervical spine injury in children suffering blunt trauma: preliminary experience with a QUICK cervical spine MR protocol. Annual meeting of the American Society of Neuroradiology, June 2011, Seattle, WA. Oral Presentation (Bardo main presenter, Guillaume first author)

Abstracts (published in electronic or paper format that are archived or searchable)

  1. Guillaume DJ, Thompson EM, Muldoon L and Neuwelt EA. Assessing vascularity of medulloblastoma using dynamic magnetic resonance imaging. American Society of Pediatric Neurosurgery Annual Meeting, January 31, 2011, Lanai, Hawaii. Oral Presentation. Journal of Neurosurgery – Pediatrics, Jan 2011.
  2. Steiner RD, Huhn SL, Koch T, Al-Uzri A, Guillaume D, Sutcliffe T, Vogel H, Selden N. CNS transplantation of purified human neural stem cells in Infantile and Late-Infantile Neuronal Ceroid Lipofuscinoses:Summary of the Phase I trial. Mol Gen Metab. 98: 76., A422, 2009.
  3. Guillaume D, Muldoon L, Lewin S, Gharamanov S and Neuwelt E. Disseminated Medulloblastoma in the Nude Rat, A Model for Testing Therapeutics. American Society for Pediatric Neurosurgeons, National Meeting, Kohala Coast, Hawaii, Jan 27, 2009. Oral Presentation. Journal of Neurosurgery – Pediatrics, Jan 2009
  4. Selden NR, Guillaume DJ, Steiner RD and Huhn SL. (2009) CNS Transplantation of Purified Human Neural Stem Cells in Subjects with Infantile and Late-Infantile Neuronal Ceroid Lipofuscinosis: A Phase I Trial. American Society for Pediatric Neurosurgeons, National Meeting, Kohala Coast, Hawaii, Jan 28, 2009. Oral Presentation. Journal of Neurosurgery –Pediatrics, Jan 2009
  5. Guillaume D, Muldoon L, Lewis S, Gharamanov S and Neuwelt E. (2008) Characterization of a disseminated medulloblastoma model, MO-10,Neuro-oncology,vol 10, p 848
  6. Guillaume D and Zhang SC (2003). In vivo differentiation of human ES cell-derived neural precursors. Society for Neuroscience Annual Meeting Publications.
  7. Guillaume D and Zhang S. Differentiation of human embryonic stem cell-derived neural precursors following transplantation into the immunodeficient mouse brain (2003) Journal of stereotactic and functional neurosurgery. 



Neurosurgery Clinic;Pediatric Specialty Care-Explorer Clinic;University of Minnesota Masonic Children's Hospital

Board Certifications

  • National Board of Medical Examiners; Minnesota, 2017
  • Certified Diplomat of the American Board of Pediatric Neurosurgery, 2016
  • Certified Diplomat of the American Board of Neurological Surgery, 2010