Daniel J. Guillaume, MD, MS

Associate Professor, Department of Neurosurgery

Daniel J. Guillaume

Contact Info


Mailing Address:
MMC 96
420 Delaware St. SE
Minneapolis, MN 55455

Administrative Assistant Name
Deborah Fellows

Administrative Phone

Administrative Email

Associate Professor, Department of Neurosurgery


University of Iowa Carver College of Medicine

University of Iowa Hospitals and Clinics (Neurosurgery)

Prince of Wales Hospital and Sydney Children's Hospital, (Pediatric and Minimally Invasive Neurosurgery)

University of Iowa (Neurosurgery)

University of Wisconsin (Stem Cell Research)


After completing his neurosurgery residency at the University of Iowa Hospital and Clinics, Dr. Guillaume completed two clinical fellowships. The first was at the University of Iowa where he worked with Dr. Arnold Menezes, a pediatric neurosurgeon and world expert in development and pathologies of the cranial base and craniovertebral junction. He then completed a fellowship at the Centre for Minimally Invasive Neurosurgery, Prince of Wales Hospital and Sydney Children's Hospital in Sydney, Australia with Dr. Charles Teo, a pediatric neurosurgeon and pioneer in minimally invasive approaches to the brain. These approaches allow treatment of problems, including hydrocephalus and brain tumors, through a tiny incision, often leading to less pain and a shorter hospital stay after surgery. In many cases, these approaches can successfully treat hydrocephalus with avoidance or removal of shunt catheters, and some brain tumors can be removed through the nose or eyebrow avoiding the need for large scalp incisions.

Through his unique training, Dr. Guillaume has developed expertise for managing pediatric brain tumors and hydrocephalus, Chiari malformation, and pediatric skull base surgery to name a few. He joined the University of Minnesota in 2012 after practicing pediatric neurosurgery for almost six years as a faculty member at Oregon Health & Science University in Portland. He has developed experience in caring for children with all types of congenital and acquired neurosurgical problems.

Dr. Guillaume is currently Chief of Pediatric Neurosurgery at the University of Minnesota Masonic Children's Hospital and Director of the Division of Pediatric Neurosurgery for the Department of Neurosurgery. His interests include advanced treatments of pediatric brain and spinal tumors, minimally invasive surgical approaches, complex hydrocephalus, and Chiari malformation. He has published in numerous journals and books, directed courses on minimally invasive and endoscopic surgical approaches, served as reviewer and co-editor for major neurosurgery and stem cell journals and served as a moderator and scientific program committee member for national pediatric neurosurgical meetings.

Dr. Guillaume was driven toward pediatric neurosurgery because he enjoys caring for children and their families, and is committed to improving the lives of children suffering from neurological problems.

Awards & Recognition

  • Mpls/St. Paul Magazine’s Top Doctors, 2013, 2014, 2015, 2016
  • Best Doctors in America, 2011-2012; 2013, 2014, 2015
  • Best Oregon Doctors, Portland Monthly Magazine, Dec. 2006, 2007, 2008
  • SuperDocs, 2013, 2014
  • Top Neurosurgeon in Minneapolis, MN, International Association of Healthcare Professionals (The Leading Physicians of the World), 2014, 2015


Research Summary/Interests

Research Interests

Dr. Guillaumes’s research is focused on improving diagnosis and therapy for pediatric brain tumors by use of advanced imaging, and efforts to improve delivery of anti-tumor agents by manipulation of the blood-brain barrier. His other ongoing research studies evaluate methods to improve treatment of hydrocephalus using image guidance, and investigate the association of hearing loss in treatments for hydrocephalus.

Research Projects

  • Evaluation of hearing loss in children after treatment for hydrocephalus, Funded by the Capita foundation
  • Study of treatments for Chiari I malformation with syringomyelia (member of the Park-Reeves Syringomyelia Research Consortium), funded by the Park-Reeves Foundation
  • Imaging vascular properties of pediatric brain tumors using DSC-MRI with ferumoxytol (Code 7228) and DCE MRI with gadolinium in a single imaging session: An NCI sponsored exploratory trial, Funded by NIH (OHSU, Portland, OR).
  • A Randomized, Controlled, Multicentre, Phase IIb Safety and Efficacy Study of HGT-1410 (Recombinant Human Heparan N Sulfatase) Administration via an Intrathecal Drug Delivery Device in Pediatric Patients with Early Stage Mucopolysaccharidosis Type III A Disease, Jan 2015 to present, Role:Co- investigator (PI- C Whitley), agency: Shire
  • AAVrh.10-hMPS3A, an adeno-associated viral (AAV) vector encoding the human N-sulfoglycosamine sulphohydrolase (SGSH) gene, Jan 2015 to present, Role:Co- investigator (PI- C Whitley), agency: Lysogene



  • Das P and Guillaume DJ. Targeting the RAS-ERK signaling pathway as therapy for pediatric brain tumors: A review. Journal of Pediatric Oncology, 2015, In Press
  • Satzer D and Guillaume DJ. Hearing loss in hydrocephalus: a review, with focus on mechanisms. Neurosurgical Review, In Press
  • Satzer D and Guillaume D. Prognostic Value of Newborn Hearing Screening in Myelomeningocele. J Neurosurgery – Peds, 2014 Nov;14(5):4950500, PMID: 25216288
  • Skrpek M, Foreman N, Guillaume D, and Moertel C. Pilomyxoid astrocytoma treated successfully with verurafenib. Pediatr Blood Cancer 2014 May 12, PMID 24821190
  • Das P, Goyal T, Xue Z, Kalatoor S and Guillaume D. Simulation Training in Neurological Surgery. Austin J. of Neurosurgery. 1(1):1-6, 2014
  • Bergmann K, McCabe J, Smith T, Guillaume D, Sarafoglou K and Gupta S. Late-onset ornithine transcarbamylase deficiency: treatment and outcome of hyperammonemic crisis. Pediatrics 133(4):749-750, www.pediatrics.org, PMID 24616362
  • Hunt MA, Guillaume DJ. "Potential of Ferumoxytol as an Intraoperative Contrast Agent and for Imaging Adult and Pediatric Brain Tumors." Am J Neuroradiol News Digest 2013 Aug
  • Selden NR, Al-Uzri A, Huhn SL, Koch TK, Dikora DM, Nguyen-Driver MD, Guillaume DJ, Koh JL, Gulktekin SH, Anderson JC, Bogel H, Sutcliffe TL, Jacobs Y, Steiner RD. Central nervous system stem cell transplantation for children with neuronal ceroid lipofuscinosis. J Neurosurg: Pediatr. 2013 Jun;11(6)643-52, 2012, PMID: 23581634.
  • Piedra M, Thompson EM, Selden NR, Ragel B and Guillaume D. Optimal timing of autologous cranioplasty after decompressive craniectomy in children, J Neurosurg: Peds, 10(4):268-272, 2012, PMID: 22861195.
  • Thompson EM, Guillaume DJ, Dosa E, Li X, Nazemi KJ, Hamilton BE, Neuwelt EA. Dual contrast perfusion MRI in a single imaging session for assessment of pediatric brain tumors, J Neuro-Oncology, 2012, PMID: 22528798.
  • Guillaume DJ, Knight K, Marquez C, Bardo D, Kraemer D, Neuwelt EA. Cerebrospinal fluid shunting and hearing loss in patients treated for medulloblastoma, J Neurosurg: Peds, 9(4):421-427, 2012, PMID: 22462709.
  • Dosa E, Guillaume DJ, Haluska M, Lacey CA, Hamilton BE, Njus JM, Rooney WD, Kraemer DF, Muldoon LL, Neuwelt EA. Magnetic resonance imaging of intracranial tumors: intra-patient comparison of gadoteridol and ferumoxytol, Neuro-oncology, 13(2)251-260, 2011. PMID: 21163809.
    Raskin J, Guillaume D and Ragel B. Laparoscopic induced pneumocephalus in a patient with a ventriculoperitoneal shunt, J Neurosurgery – Pediatrics, 46(5)390-391, 2010, PMID: 21412025.
  • Guillaume DJ. Positional Plagiocephaly, in the International Society for Pediatric Neurosurgery Guide (invited submission), 2011.
    Guillaume D. Fusion with Chiari Surgery, Chiari & Syringomyelia Foundation Website (invited submission) www.csfinfo.org/physinfo, 2010.
  • Guillaume D, Doolittle ND, Gahramanov S, Hedrick NA, Delashaw JB, and Neuwelt EA. (2010) Intra-arterial chemotherapy with osmotic blood-brain barrier disruption for aggressive oligodendroglial tumors: Results of a Phase I study, Neurosurgery 66(1).
  • Guillaume DJ and Zhang SC. (2008) Human embryonic stem cells: a potential sources of transplantable neural progenitor cells. Neurosurg Focus, 24 (3&4): E2, Mar-Apr 2008.
  • Guillaume DJ, Huhn SL, Selden NR, and Steiner RD. (2008) Cellular therapy for childhood neurodegenerative disease. Part I: Rationale and preclinical studies. Neurosurg Focus 24 (3&4): E21, Mar-Apr 2008.
  • Selden NR, Guillaume DJ, Steiner RD, and Huhn SL. (2008) Cellular therapy for childhood neurodegenerative disease. Part II: Clinical trial design and implementation. Neurosurg Focus 24 (3&4): E21, Mar-Apr 2008.
  • Guillaume DJ, Johnson MA, Li XJ, and Zhang SC. (2006) Human embryonic stem cell-derived neural precursors develop into neurons and integrate into the host brain. Journal of Neuroscience Research 84: 1165-1176.
  • Guillaume D and Menezes A. (2006) Retroclival hematoma in the pediatric population: Report of two cases and review of literature. Journal of Neurosurgery (4Suppl Pediatrics) 105: 321-325.
  • Guillaume D, Bertrand P, Dea D, Davignon J, and Poirier J. (1996) Apolipoprotein E and low-density lipoprotein binding and internalization in primary cultures of rat astrocytes: Isoform-specific alterations. Journal of Neurochemistry 66: 2410-2418.
  • Bertrand P, Guillaume D, Hellauer K, Dea D, Lindsay J, Kogan S, Gauthier S, and Poirier J. (1993) Distribution of herpes simplex virus type 1 DNA in selected areas of normal and Alzheimer's disease brains: A PCR study.Neurodegeneration 2: 201-208.

Book Chapters

  • Das P and Guillaume D. Pediatric Brain Tumors: Other, in: Neurosurgical Knowledge Update, editors: Mark Krieger and Gerry Grant, Thieme, In press
  • Guillaume DJ. Congenital Defects of the Scalp and Skull, in: Pediatric Neurosurgery Tricks of the Trade (Ed: Alan Cohen). Thieme, in press.
  • Guillaume DJ, Doolittle ND, Neuwelt EA. Oligodendroglial Tumors: Intra-arterial chemotherapy, in “Tumors of the CNS”, E. Hayat (ed), In Press, 2011.
  • Guillaume DJ. Minimally invasive neurosurgery for CSF Disorders, in Neurosurgery Clinics of North America, 21(4)653-672, 2010, PMID: 20947034.
  • Guillaume DJ and Menezes AH. Hydrocephalus in Chiari malformation and other craniovertebral junction abnormalities, in Mallucci C and Sgouros S (editors) “Cerebrospinal Fluid Disorders” Informa Healthcare, NY; pp 300 – 317, 2009.
  • Guillaume DJ and Teo C. Endoscopic management of specific disease entities, in Mallucci C and Sgouros S (editors) “Cerebrospinal Fluid Disorders” Informa Healthcare, NY; pp 508 – 527, 2009.
  • Guillaume DJ. Chiari Malformation and Syringomyelia, Pediatric Neurosurgery, Iskandar B (ed) (educational series of the an Arab Journal of Neurosurgery), MSD Printing Press, pp 238 – 258, 2008.
  • Guillaume D, Haddad F, Haddad G and Hitchon P. Diagnosis and management of traumatic intracranial hemorrhage, in Schmidek H and Roberts D (editors) “Operative Neurosurgical Techniques” 5th ed, pp 1224 – 1232, 2005.
  • Guillaume D and Hitchon PW. Medical and surgical management of intracerebral hemorrhage, in Adams HP (editor) “Handbook of cerebrovascular disease,” 2nd edition pp 477-5000 (chap 21), 2004.
  • Guillaume D, and Zhang SC. Neuronal replacement by transplantation, in Bottenstein JE (editor) “Neural Stem Cells: Development and Transplantation,” page 307-338, 2003.
  • Guillaume D, Karanjia PN, and Hitchon PW. Traumatic intracranial aneurysms. Medlink Neurology (online information resource for clinical neurology), www.medlink.com, 2003.
  • Guillaume DJ and Hitchon P. Spontaneous intracerebral hemorrhage, in Sinha KK (editor), “Advances in Clinical Neurosciences”, 13th ed, p 501-516, 2003.
  • Guillaume DJ, Haddad GF and Hitchon P. Post-traumatic intracranial aneurysms, in Sinha KK (editor), “Advances in Clinical Neurosciences”, 13th ed, p 517-526, 2003.
  • Guillaume D, Bertrand P, Dea D, Davignon J, and Poirier J. Low-density lipoprotein pathways in the central nervous system and apolipoprotein E isoform-specific differences. In: Research Advances in Alzheimer’s disease and Related Disorders. (K. Iqbal, J.A. Mortimer, B. Winblad and H.M. Wisniewski, eds), John Wiley & Sons Ltd. P385-395, 1995.
  • Poirier J, Nalbantoglu J, Guillaume D, and Bertrand P. Apolipoprotein E and Alzheimer’s disease. In: Pathobiology of Alzheimer’s Disease (Goate A. editor), Academic Press. Chapter 41, 1995.

Other Published Articles




Neurosurgery Clinic;Pediatric Specialty Care-Explorer Clinic;University of Minnesota Masonic Children's Hospital

Board Certifications

American Board of Medical Specialties (ABMS) Neurological Surgery